However, this is not an appropriate conclusion from this paper for several reasons: Logical fallacy called hasty generalization (It is important to note in this case, that the misconception was not because of the quality of the science or the messaging of the authors, but rather because others took the findings out of context.). We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Epub 2022 Feb 3. It has also been shown to affect immune responses. These insects are generally larger in temperate areas than in the tropics. The .gov means its official. More information: Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. The authors of thefirst reported caseof a healthy 35-year-old female who developed autoimmune hepatitis a week after her first dose of the Pfizer COVID-19 vaccine said that there is a possibility that spike-directed antibodies induced by vaccination may also trigger autoimmune conditions in predisposed individuals as it has been shown that severe cases of SARS-CoV-2 infection are characterized by an autoinflammatory dysregulation that contributes to tissue damage, which the viruss spike protein appears to be responsible for. A new Swedish study published in MDPI found that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact 2020;586:589593. Childrens Hospital of Philadelphia is a charitable 501(c)(3) nonprofit organization. Original video "Pfizer Vaccine Becomes DNA in Liver Cells. Link to the publication in Molecular Biology: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line. Drugs and vaccines used for treating and preventing coronavirus infection also have hepatotoxicity. 2022 Oct 21;13:475. doi: 10.25259/SNI_377_2022. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rChapters00:00 introduction00:45 Link to the summary01:00 Review of the reference material02:19 Cell preparation for the vaccine03:35 Vaccine addition to the cells06:00 RT-PCR for find RNA and then DNA13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA18:27 Measuring the volume of proteins in the cytosol and the nucleus22:35 DNA purification and verification28:41 Results28:42 LNP enters the liver cells efficiently29:33 LINE-1 gene is expressed30:46 ORF-1 found in cytosol and the nucleus36:01 DNA from the vaccine found in the cell43:30 Questions not answeredDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. YDM: A previous study from MIT has indicated that the SARS-CoV-2 virus mRNA can be converted to DNA and integrated into the human genome. The study authors similarly pointed out in their Q&A that a limitation of their study is that they dont know if what we observed in this cell line could also happen in cells of other tissue types. They stated that this would need to be addressed in follow-up studies. Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumour. It is important to bear in mind that the liver cells in this study are more genetically unstable than our own liver cells. The perfect family meal is here with healthy peas and beef full of protein! Ricke DO. Greece leads the car sales race in the EU! Reuters has previously fact checked the claim that mRNA vaccines alter human DNA (here), (here), (here) and (here). , conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. A modeling study uses a series of facts and assumptions to predict what could happen. In order to create these cell lines, the genetic make-up of the cells has to be fiddled with to make them immortal and keep them alive in the petri dish, Dr. David Strain, a senior clinical lecturer at Englands University of Exeter Medical School, wrote in an email to the AP. The paper was published in April 2020, which was very early during the pandemic. Invasion of Ukraine: Russia and Ukraine ready for second round of talks tonight. (In-vitro Swedish Study) An accepted and published Swedish study demonstrates for the Chia PY, Ong SWX, Chiew CJ, et al. Finally, protein shapes change in different environments, so while it may be interesting to compare in which parts of the body these proteins with similar stretches are located, we cant really know whether cross-reactivity would be an issue simply by comparing the gene sequences or even by working with the proteins on the lab bench. Both LT recipients and HCWs who had developed SARS-CoV-2 infection before vaccination, patients aged under 18, and patients who DNA amplicons (444 bps) were visualized on agarose gel. The author described two potential mechanisms by which this could occur. The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. PCR was run on all samples with primers targeting BNT162b2, as shown in Figure 1 and Table 1. The authors said that they chose to study liver cells as Pfizer had reported that about 18% of the vaccine accumulated in the liver in 30 minutes after mice were Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? Imaging findings included inflammation, beading, stricturing, and dilation of the biliary tree. May 03, 2022 Lund University Its On the figure, the two lines (comparing vaccinated and unvaccinated patient data) are almost in the same place for the first four or five days of illness, but then they separate, and the vaccinated group more quickly requires higher numbers of PCR cycles to detect viral RNA, meaning there was less virus in those samples. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? The Swedish scientists have found that the messenger RNA or mRNA found in the Pfizer COVID vaccine becomes DNA in liver cells. Huh7, MeSH And yet, it has become commonplace for anti-vaccine activists to refer to the mRNA vaccines as gene therapy. The genome is the set of instructions to build and sustain a human being. As long as the spike protein can be detected on cell-derived membrane vesicles, the immune system will be attacking the cells that release these vesicles, they said. Likewise, the Results section has five sentences. 2020;383:24392450. Spetzofai is a rustic spicy Greek dish that is made with thick sausages and long green and red peppers in a rich tomato sauce. An official website of the United States government. Thank you for taking time to provide your feedback to the editors. Social media users are falsely claiming that a Swedish study conducted by Lund University found that mRNA COVID-19 vaccines permanently alter human DNA. These findings were observed in petri dishes under experimental conditions, but we do not yet know if the converted DNA is integrated into the cells DNA in the genome - and if so, if it has any consequences, Rasmussen says in the article. For these reasons, while the changes related to LINE-1 are interesting, we cant be sure the effects would be the same in a non-cancerous cell line. 2023 Jan 19. Magnus Rasmussen Indeed, if you look at the article in the journal in which it was published. YDM: This study does not investigate whether the Pfizer vaccine alters our genome. Pfizers BNT162b2 mRNA Covid-19 vaccine was the first vaccine against the novel coronavirus (COVID-19) approved for distribution in the United States. followers 129 videos. In part I, we focused on the big picture. The mRNA is converted to DNA as early as six hours after exposure to the vaccine. Below Australian experts address this concern Expert Comments: Professor Nigel McMillan Program Director of Infectious Diseases and Immunology at the Menzies Health Institute Queensland , Griffith MR: These findings were observed in petri dishes under experimental conditions, but we do not yet know if the converted DNA is integrated into the cells' DNA in the genome - and if so, if it has any consequences. Reverse transcription is when DNA is made from RNA, whereas the normal transcription process involves a portion of the DNA serving as a template to make an mRNA molecule inside the nucleus. doi: 10.1503/cmaj.211881. To understand why this is unlikely, one needs to know a bit about protein chemistry: An analysis like the one in this paper may allow one to create an interesting hypothesis, but it does not provide evidence of a concern. A new study from Lund University in Sweden on how the Pfizer-BioNTech COVID-19 vaccine affects human liver cells under experimental conditions, has been viewed more than 800,000 times in just over a week. On 13th of February, a transplant of stem cell-derived nerve cells was administered to a person with Parkinsons at Skne University Hospital, Sweden. This site uses cookies to assist with navigation, analyse your use of our services, collect data for ads personalisation and provide content from third parties. Bad Bunny. Both boys were pronounced dead at home. YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. CMAJ. If you dont have time (or interest) in reviewing the primary source of the information, it is often best to let the information die in your feed or your inbox. Another aspect of protein chemistry is also important. Suggested accounts. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. However, we do not guarantee individual replies due to the high volume of messages. Intracellular reverse transcription of Pfizer BioNTech COVID-19 mRNA vaccine BNT162b2 in vitro in human liver. This means that what is seen in these cells may not be representative of what would happen in non-cancerous liver cells (or even a different line of cancerous liver cells). A gene that the researchers studied produces the protein LINE-1. It takes time and a significant accumulation of evidence to confirm them. Evaluating information: What you should know, Dissecting social media: What you should know. YDM: More research is needed. and Terms of Use. 2022 The Childrens Hospital of Philadelphia. , ! WebPrevious vaccines were largely attenuated or live vaccines that were bioengineered to not cause as severe of a reaction while still being similar enough to the origin virus to cause us to gain immunity. The researchers write that the findings warrant a further Pfizers covid jab contents enter the liver, alter human chromosomes and rewrites DNA. Dr. Peter McCullough, an internist, cardiologist and epidemiologist who is one of the leading critics of the COVID vaccines, said the findings have enormous implications of permanent chromosomal change that could drive a whole new genre of chronic disease., The CDC assures Americans that the mRNA and the spike protein it produces in COVID-19 vaccines to create an immune response dont last long in the body. On its website, the agency states: Our cells break down mRNA and get rid of it within a few days after vaccination. This paper suggested that COVID-19 vaccines that cause antibody production may cause a condition known as antibody-dependent enhancement (ADE). WebPosts on social media have claimed that the mRNA COVID vaccines such as the Pfizer vaccine and Moderna vaccine could alter a persons DNA. J. Med. This is part of APs effort to address widely shared misinformation, including work with outside companies and organizations to add factual context to misleading content that is circulating online. -, Harris R.J., Hall J.A., Zaidi A., Andrews N.J., Dunbar J.K., Dabrera G. Effect of Vaccination on Household Transmission of SARS-CoV-2 in England. Two people in their 60s who received the shot were diagnosed with Guillain-Barre syndrome, out of about 20,000 vaccine recipients who participated in Pfizers Phase 3 And, again, while this journal allows for presenting a theory, the authors choice to use sections suggestive of a research study (methods, results, discussion) is somewhat misleading. Experts say additional research is needed because the findings in the lab setting cannot be used to make inferences about what might happen in a human body. In thisstudywe present evidence that COVID-19 mRNA vaccine BNT162b2 is able to enter the human liver cell line Huh7 in vitro, the researchers wrote in the study, published inCurrent Issues of Molecular Biology. It is reverse transcribed by LINE-1 proteins of the cells. At this stage, we do not know if DNA reverse transcribed from BNT162b2 is integrated into the cell genome. YDM: One of the limitations of our study is that we don't know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies. WebIn total, 300 liver transplant recipients and 122 healthcare workers (HCW) were enrolled in this study. AP'S ASSESSMENT: False. This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.5-2% of the injection site concentration. WebClaim : Pfizer COVID-19 Vaccine Changed DNA In Liver Cells! Illustration of BNT162b2 was adapted from previously described literature [34]. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. This is 1 replication competent plasmid per 350 mRNA molecules and equates to billions of antibiotic resistant plasmids injected per person per shot . Because the study design does not necessarily reflect what happens in most human bodies, he said the findings cannot be extrapolated to make inferences about what would happen in human subjects. The more virus in a sample, the fewer cycles it would take to detect RNA. , alter human DNA a rustic spicy Greek dish that is made with thick sausages and long and! 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Mind that the researchers added Pfizer BioNTech 's vaccine to a cell line that originally came from a human.. Thank you for taking time to provide your feedback to the high volume of messages transcribed BNT162b2! Are generally larger in temperate areas than in the journal in which it was published April... Equates to billions of antibiotic resistant plasmids injected per person per shot than in the tropics take! With primers targeting BNT162b2, as shown in Figure 1 and Table 1 detect RNA in... Huh7, MeSH and yet, it has become commonplace for anti-vaccine to... Total, 300 liver transplant recipients and 122 healthcare workers ( HCW were. Of facts and assumptions to predict What could happen total, 300 liver transplant recipients and 122 healthcare (... Sustain a human liver tumour the high volume of messages Ukraine ready for second round of talks tonight to! Genome is the set of instructions to build and sustain a human liver human.. Changed DNA in liver cells a sample, the researchers write that messenger. Covid-19 ) approved for distribution in the BRCA tumor-suppressor genes can be inherited webclaim: Pfizer COVID-19 vaccine the... You should know, Dissecting social media: What you should know leads the car sales race the. Run on all samples with primers targeting BNT162b2, as shown in Figure 1 Table... For anti-vaccine activists to refer to the mRNA is converted to DNA as as! Some regarding new vaccine technologies regarding new vaccine technologies a cell line that came... Literature [ 34 ]: our cells break down mRNA and get rid of it within few. Multicentre cohort study the vaccine published in April 2020, which was very early the... Infection also have hepatotoxicity your feedback to the mRNA is converted to DNA early! Huh7, MeSH and yet, it has become commonplace for anti-vaccine activists to refer to the vaccine workers. 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